Denville, NJ (PRWEB) April 02, 2014
Diagenode, Inc., a leading global provider of shearing technologies and complete solutions for epigenetics research, recently launched its Megaruptor DNA fragmentation instrument, part of its shearing technologies product line. A number of applications for massively parallel sequencing, also known as Next-Generation Sequencing, require libraries of long DNA segments, but few methods have been available for reliably generating fragments greater than 2 kb.
The Megaruptor, which has been used by several sequencing centers worldwide to produce insert fragment libraries for Illumina®, Ion TorrentTM, and PacBio® platforms, utilizes mechanical shearing to generate libraries between 2 kb and 75 kb. The instrument’s unique shearing component eliminates clogging issues frequently encountered with single orifice shearing devices and includes a disposable element that reduces the potential for cross-contamination.
A common application that requires long insert paired-end DNA libraries is mate pair library preparation, useful for de novo sequencing, detecting structural variants, and identifying rearrangements, duplications, and repeats in the genome. In a collaborative effort with Wisconsin-based Lucigen Corporation, Diagenode has successfully prepared 2 – 50 kb inserts using the Megruptor with Lucigen’s new mate-pair library technology.
Lucigen CEO stated, “In conjunction with Diagenode, we have developed a long span mate-pair technology that has demonstrated greater than 90 % efficiency for 2 to 20 kb libraries. In the future, we will be able to create 50 kb true mate-pair libraries which will enable proper assembly of many de novo genomes. Mead further elaborated, “The Megaruptor is our choice for shearing due to the instrument’s ability to function without clogging and the wide range of shearing sizes available.”
“Diagenode has been a leader in shearing solutions and devices for almost 15 years,” said Didier Allaer, CEO of Diagenode. “The Megaruptor now enables reliable long fragment shearing unmatched by other existing solutions.”
More information on Diagenode’s Megaruptor can be found at http://www.diagenode.com/en/landing-pages/megaruptor.php.
About Diagenode, Inc.:
Diagenode is a leading provider of complete solutions for epigenetics research and sample preparation. The company has developed both shearing solutions for a number of applications as well as a comprehensive approach to gain new insights into epigenetic studies. The company offers innovative shearing and automation instruments, reagent kits, and high quality antibodies to streamline DNA methylation, ChIP, and ChIP-seq workflows. The company’s latest innovations include a unique, full automation system, the industry’s most validated antibodies, and the Megaruptor shearing system for long fragment generation in sequencing. For more information about Diagenode, please visit the company’s website at http://www.diagenode.com.